Mass photometry IPBS-Toulouse,Seminar room, 1 décembre 2020-1 décembre 2020, Toulouse.

Mass photometry
IPBS-Toulouse, Seminar room, le mardi 1 décembre à 11:00
### James Wilkinson

**Refeyn Ltd., Oxford, UK**

Mass photometry

Mass photometry is an ultra-sensitive microscopy technique, which allows mass determination at the single molecule level. It is based on detecting scattering signal (contrast) generated by an object at the glass-water interface. Since mass photometry contrast is directly proportional to molecular mass it gives direct information on mass distribution with unprecedented sensitivity.

During the brief introduction I will explain the principles underlying mass determination using light, present current examples and discuss further applications including the use of the system for optimization of Cryo-EM measurements and the formation of multicomponent complexes (for example ribosomes).

**Selected references**
• Young et al. Quantitative mass imaging of single biological macromolecules **Science** (2018) 360(6387):423–427

• Sonn-Segev et al. Quantifying the heterogeneity of macromolecular machines by mass photometry **Nat Commun** (2020) 11, 1772

• Olerinyova et al. Mass photometry of membrane proteins **bioRxiv** (2020) 02.28.969287; doi: [](

• Wu & Piszczek. Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry **Anal Biochem** (2020) 592:113575

• Li et al. Single molecule mass photometry of nucleic acids Nucleic Acids Res (2020) 48(17):e97

A technology seminar by Dr. James Wilkinson, Refeyn Ltd (Oxford, UK). He will present their recent Mass Photometry technology, which allows mass tracking on single molecules.

IPBS-Toulouse,Seminar room 205 Route de Narbonne, 31400 Toulouse Toulouse Sud-Est Haute-Garonne

Dates et horaires de début et de fin (année – mois – jour – heure) :
2020-12-01T11:00:00 2020-12-01T12:00:00